Culturing Phyto on a Petri Dish

[Fizzman]

I have a question when it comes to dosing though. I've read that dosing phyto is like adding phosphates to your tank. I mean I think that's true if u add so much but at the dilution of what we dose, the cells would actually stay alive if it wasn't eaten right? So unless something eats it and process it into poop it wouldn't hurt the tank right? So yeah it eventually contributes to tank parameters but a lot better than adding stuff like oyster feast and any other type of food that will contribute to parameters without actually getting consumed other than by bacteria. Is that a correct understanding or would the phyto die pretty fast in the tank even if not eaten?

[Bigpapii]

I have a question when it comes to dosing though. I've read that dosing phyto is like adding phosphates to your tank. I mean I think that's true if u add so much but at the dilution of what we dose, the cells would actually stay alive if it wasn't eaten right? So unless something eats it and process it into poop it wouldn't hurt the tank right? So yeah it eventually contributes to tank parameters but a lot better than adding stuff like oyster feast and any other type of food that will contribute to parameters without actually getting consumed other than by bacteria. Is that a correct understanding or would the phyto die pretty fast in the tank even if not eaten?

Nah sorry I don't have pods isolated in a culture
Just think of phyto as fish food if you add too much it will add phosphate. The reason is cuz phyto won't live as long as it can in a culture. It doesn't have enough nutrients to survive and multiply. The longest it will live would be about a month or so if it doesn't get eaten and when it dies it'll turn into phosphate. Just know how much your tank needs and you should be fine. I've ven dosing for a couple months now and my phosphates are low.


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[Bigpapii]

Agar came in. Still waiting for Petri dishes...



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[Bigpapii]

Experimenting with the agar f/2 medium
This medium contains
200ml of saltwater
1ml of f/2
1ml of fluval trace elements
5g of agar powder



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[Bigpapii]

Since the Petri dishes haven't arrived I will be testing out the agar medium in medical collection tubes



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[Bigpapii]

I decided to test the medium in the Erlenmeyer flask itself because I got lazy ahah



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[Bigpapii]

Day two

Day three




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[Fizzman]

Is agar agar from the Asian store the same? How much did u pay for your agar? https://www.amazon.com/Telephone-Pro.../dp/B01KMHY2OU this stuff at the Asian supermarket is only a dollar or so, maybe 2 max. It's food so it's the same right?

[Bigpapii]

Is agar agar from the Asian store the same? How much did u pay for your agar? https://www.amazon.com/Telephone-Pro.../dp/B01KMHY2OU this stuff at the Asian supermarket is only a dollar or so, maybe 2 max. It's food so it's the same right?

Yeaa it's the same thing. It's a lot cheaper if you buy it there. The Asian market I go to was out of stock so I had to order it online


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[Bigpapii]

Petri dishes came in. I made the agar medium and filled them up. Gonna let it cool down over night and seed them tomorrow morning
Im starting off with just 5 cultures for now.



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[Bigpapii]

I was impatient so I decided to cool it in the fridge. Then seeded them with nannochloropsis from the flask.


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[Bigpapii]

Update on the flask

Some leaked through the agar and sits at the bottom of the bottle and started reproducing



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[Nick Shades]

Very interesting. I cannot wait to see how this takes off.

[Fizzman]

Agar is made from seaweed, so putting it into the tank will add some nutrients, the question is, how are you going to determine population density to know how big of a pie slice to give the tank. Also, will you be testing the amount of f/2 left(like how do you know when the culture has used up most of its food source) because adding straight f/2 to the tank is probably bad, algae bloom for sure.

[Fizzman]

Although it would be cheap to ship it as a starter culture, or even as a rehydrate before use product.