What Robert said.
I added my livestock about 24 hours later. Fish and coral. I did buy a oversized bottle and continued to add capfuls of dr.Tims over the following week.
Just don't pour the bottle in and wait too long without adding some kind of bioload. The bacteria needs something to feed off and stay alive. whether it be old tank water, old sand or rock or skim like stated above.
Also what size tank and how many fish are we talking here? All new dry mined rock and sand? or established rocks and sand? I had never had detectable ammonia, just a very small amount of nitrite for a day, then it was all nitrate from there on out. I used some of that mined base rock the sell on MD, so there was no major die off from the rocks and the sand was rinse really well.