Hi, yeah there were about 20 other fish in the tank. I think what got them going was the ROE eggs I was feeding the Anthisis trying to get them to feed correctly. The tank was overwhelmed with food and that I understand helps trigger the response. My tank is way oversized for the population (total 420 gallons) and I'm running a skimmer, two reactors and a 90g fuge) but still I started having water issues.